What is Nettle Extract?Nettle extract is the dry whole plant extract of Urtica urtica Urticacannabina L., Urtica angustifolia UrticalaetevirensMaxim., Urtica angustifolia Fisch.exHornem. It mainly contains flavonoids, lignans, and steroids. , Lipids, organic acids, proteins, tannins, chlorophyll, alkaloids and polysaccharides. It has biological activities such as anti-rheumatic, lowering blood sugar and treating benign prostatic hypertrophy.
Human colon-cancer cells (SW480) were provided as a gift by the Rudjer Boskovic Institute, Zagreb, Croatia. Cells were grown as monolayer cultures in DMEM (GIBCO, USA), supplemented with 10 % of the foetal bovine serum (GIBCO, USA), 4500 mg/L of glucose and 1 % of penicillin/streptomycin. The final concentration of nettle extract obtained for the experiments on SW480 cells (10 g of plant material in 200 mL of water), expressed as the extraction yield, ranged from 17.5 to 176 g/L (concentrations provided for the range of 0.5x – 5x).
Cytotoxicity of the final nettle extract (Fig. ) obtained after the extraction comparisons, was in dependence of the extract concentration and treatment duration (1 or 2 h). The treatment of SW 480 cells caused a dose–response decrease in cell survival during both 1 and 2 h of incubation. The cytotoxic effect of the extract was exerted by higher nettle extract concentrations (2.5x and 5x), while prolonged incubation (2 h) with the highest extract concentration (5x) caused a lower but insignificant decrease in cell survival (in comparison to 1 h treatment). After 2 h of incubation with nettle extract (5x), the cell survival exhibited only 32 %, indicating the cytotoxic effect of it on SW480 colon cancer cell line. The recovery period of 24 h (denoting one cell cycle period) had no significant effect (p < 0.05) on the cell survival of SW 480 cells.Basic Information:ANALYSISSPECIFICATIONRESULTSMethodAppearanceYellow brown powderCompliesOrganolepticOdorCharacteristicCompliesOrganolepticAssay(by)
Sieve analysis100% pass 80 meshComplies80 mesh ScreenLoss on Drying≤5.0%3.51%Eur.Ph.7.0<2.2.32>ICP-MSSulphacted Ash≤5.0%3.06%Eur.Ph.7.0<2.2.32>ICP-MSHeavy Metal<10ppmCompliesEur.Ph.7.0<2.2.58>ICP-MSAs<2ppmCompliesEur.Ph.7.0<2.2.58>ICP-MSHg<2ppmCompliesEur.Ph.7.0<2.2.58>ICP-MSCd<2ppmCompliesEur.Ph.7.0<2.2.58>ICP-MSPb<2ppmCompliesEur.Ph.7.0<2.2.58>ICP-MSMicrobiology
Total Plate Count<10000CFU/gCompliesEur.Ph.7.0<2.6.12>ICP-MSYeast & Mold<1000CFU/gCompliesEur.Ph.7.0<2.6.12>ICP-MSE.ColiNegativeNegativeEur.Ph.7.0<2.6.12>ICP-MSSalmonellaNegativeNegativeEur.Ph.7.0<2.6.12>ICP-MSStaph-aureusNegativeNegativeEur.Ph.7.0<2.6.12>ICP-MS
Nettle Extract Benefits:1.Soothes allergiesIn its natural state, the stinging nettle root extract stimulates an allergic reaction when its fine hairs touch the skin. However, when prepared as tea, nettles may actually help soothe allergies. A study published in 2017 in the Iranian Journal of Pharmaceutical Research suggests that nettles can help relieve the symptoms of hay fever. Here are other natural remedies that can help you relieve allergy symptoms without relying on medication so you can avoid sniffling and sneezing through the entire season.2.Treats muscle and joint pain
Because of its natural anti-inflammatory and analgesic (aka pain-relieving) properties, nettles have been used for centuries to treat sore, stiff muscles and joints. Nettles may help alleviate the symptoms of osteoarthritis and joint pain, typically in the case of hands, knees, hips, and spine, according to a review of research published in 2018 in the journal Molecules. In the review, researchers also found that nettles can work in combination with nonsteroidal anti-inflammatory drugs (NSAIDs), allowing people to decrease their use of NSAIDs. An anti-inflammatory diet may also reduce pain and keep inflammation in check. Make sure you have these staples of a pain- and inflammation-fighting diet.3.Strengthens bones
Nettle extract flours deliver bone-fortifying calcium, magnesium, and iron, according to research, including a study published in 2016 in Food Science & Nutrition. The plant also contains vitamins D and K, which help bones use calcium to protect bone density. Magnesium is stored in the bones, supports bone strength, and aids in the absorption of calcium. Low magnesium is linked to osteoporosis, a chronic condition of weak and fragile bones. Iron deficiency is also associated with bone loss. Get to know these other, less well-known bone conditions that can cause pain, limit mobility, and interfere with daily life.4.Boosts heart health
Nettles contain vitamins and minerals that are regarded as heart protectors. The plant is a source of vitamins A and C, beta carotene, and other carotenoids. Because of its high content of vitamin C and iron, which help the body boost red blood cell production, it may help prevent anemia. Nettles also contain the flavonoids quercetin and kaempferol, which can help lower blood pressure and reduce the risk for cardiovascular disease. A source of iron and potassium, tea made from this spiky, leafy plant may help to relax blood vessels and aid in healthy circulation. Check out these updated guidelines for treating high blood pressure.5.Soothes skin irritations
Thanks to the plant’s antihistamine, anti-inflammatory, anti-microbial, and pain-relieving abilities, nettle has a long history of being used to treat skin irritations like eczema and acne, says Jeanette Jacknin, MD, a holistic dermatologist in Solana Beach, California, and author of Smart Medicine for Your Skin. It seems to reduce redness and swelling, soothe itching, and speed healing. To get these skin-clarifying benefits, you can drink the tea—or you can brew a cup, let it cool, and apply the liquid directly to your skin. However, cautions Dr. Jacknin, “there are reports of allergic skin reactions to topical nettle, so use on just a small area of skin first to make sure you don’t have any skin sensitivity.”6.Boosts kidney, urinary, and prostate health
As a diuretic, nettles can promote healthy urination. The stinging nettle plant is used as a natural remedy for urinary tract infections, and to prevent kidney stones. A study of rats, published in 2014 in Molecular Medicine Reports, shows that it reduced build-up of calcium oxalate crystals in the kidneys, which contribute to the formation of the most common type of kidney stones. However, findings in animal studies are not directly transferable to humans. Under the care of a physician, extracts of the plant are used to treat the urinary symptoms of enlarged prostate, including a frequent need to urinate and difficulty emptying the bladder. Know these signs of a urinary tract infection.7.Strengthens immune function
The nettle extract contains several immune-boosting compounds, including flavonoids, carotenoids, and vitamins A and C. These antioxidants help protect immune cells against damage that can weaken immune function. Research shows nettle extract strengthens the immune response, encouraging immune cell activity. Scientists at Hacettepe University in Ankara, Turkey, found that the plant stimulates the T-cells of the immune system, which help fight infection and other disease-causing pathogens in the body. Try these everyday habits to boost your immune system.8.Regulates digestion
Excessive inflammation can interfere with healthy digestion and promote the growth of harmful bacteria in your gastrointestinal tract. Regularly consuming stinging nettle tea, with its anti-inflammatory powers, may help you regulate digestion, and reduce constipation, diarrhea, and upset stomach. By keeping your gut stocked with the right balance of bacteria and other microbes—known collectively as your microbiome—you may also get protection against a much wider spectrum of diseases. Learn how maintaining a healthy gut may help you age more slowly—and keep you in better health.Application:
Stinging nettle has been used for hundreds of years to treat painful muscles and joints, eczema, arthritis, gout, and anemia. Today, many people use it to treat urinary problems during the early stages of an enlarged prostate (called benign prostatic hyperplasia or BPH).
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